PrimePCR™ Probe Assay: Gpx3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Gpx3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gpx3, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053363
Assay Design:   exonic
Chromosome Location:   11:54907276-54908635question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000125094 ENSMUST00000082430 ENSMUST00000149324

Gene Information

This gene product belongs to the glutathione peroxidase family which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This gene is highly expressed in the kidney where it may play a role in protecting the kidney from oxidative damage. [provided by RefSeq Feb 2012]

Gene Symbol:   Gpx3
Gene Name:   Glutathione peroxidase 3
Aliases:   AA960521, EGPx, GPx, GSHPx-3, GSHPx-P
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000018339
Entrez:   14778
Chromosome Mapping:   11|11 B3-B5

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