PrimePCR™ Probe Assay: Prkar1b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055187
Assay Design:   exonic
Chromosome Location:   5:139127677-139129648question
Amplicon Length:   84
Splice Variants Targeted:   ENSMUST00000026973 ENSMUST00000110890 ENSMUST00000148002 ENSMUST00000129851 ENSMUST00000147505 ENSMUST00000155833 ENSMUST00000134762

Gene Information

Description Not Available

Gene Symbol:   Prkar1b
Gene Name:   Protein kinase, cAMP dependent regulatory, type I beta
Aliases:   AI385716, RIbeta
RefSeq:   NC_000071.6 NT_039316.8
Ensembl:   ENSMUSG00000025855
Entrez:   19085
Chromosome Mapping:   5 G2|5 82.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.850000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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