PrimePCR™ Probe Assay: Cxcl12, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Cxcl12, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cxcl12, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0032307
Assay Design:   Intron-spanning
Chromosome Location:   6:117171511-117173552question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000112866 ENSMUST00000112871 ENSMUST00000073043

Gene Information

The protein encoded by this gene is a member of the intercrine-macrophage inflammatory protein superfamily. It is a secreted protein thought to be involved in the generation and proliferation of early B-cell progenitors. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   Cxcl12
Gene Name:   Chemokine (C-X-C motif) ligand 12
Aliases:   AI174028, Pbsf, Scyb12, Sdf1, Sdf1a, Sdf1b, Tlsf, Tlsfa, Tlsfb, Tpar1
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000061353
Entrez:   20315
Chromosome Mapping:   6 F1|6 53.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.800000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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