PrimePCR™ Probe Assay: Ncf1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Ncf1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Ncf1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0026937
Assay Design:   intron_spanning
Chromosome Location:   12:27505798-27507054question
Amplicon Length:   97
Splice Variants Targeted:   ENSRNOT00000002028

Gene Information

involved in the regulation of arthritis severity in rats [RGD, Feb 2006]

Gene Symbol:   Ncf1
Gene Name:   Neutrophil cytosol factor 1
Aliases:   Not Available
RefSeq:   NM_053734
Ensembl:   ENSRNOG00000001480
Entrez:   114553
Chromosome Mapping:   12q12

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