PrimePCR™ Probe Assay: HSPG2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSPG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSPG2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039323
Assay Design:   exonic
Chromosome Location:   1:22222681-22222779question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000374695 ENST00000412328 ENST00000439717

Gene Information

This gene encodes the perlecan protein which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin prolargin collagen type IV FGFBP1 FBLN2 FGF7 and Transthyretin etc. and plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g. FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1 Silverman-Handmaker type of dyssegmental dysplasia and Tardive dyskinesia.[provided by RefSeq Mar 2010]

Gene Symbol:   HSPG2
Gene Name:   heparan sulfate proteoglycan 2
Aliases:   HSPG, PLC, PRCAN, SJA, SJS, SJS1
RefSeq:   NC_000001.10 NG_016740.1 NT_004610.19
Ensembl:   ENSG00000142798
Entrez:   3339
Chromosome Mapping:   1p36.1-p34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.310000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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