PrimePCR™ Probe Assay: DAB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DAB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DAB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054240
Assay Design:   exonic
Chromosome Location:   1:57602314-57611076question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000371236 ENST00000371231 ENST00000371232 ENST00000332102 ENST00000371230 ENST00000371234 ENST00000420954 ENST00000414851 ENST00000439789

Gene Information

The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice the disabled-1 gene plays a central role in brain development directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1 and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported but their full length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DAB1
Gene Name:   disabled homolog 1 (Drosophila)
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000173406
Entrez:   1600
Chromosome Mapping:   1p32-p31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.450000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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