PrimePCR™ Probe Assay: SPARC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: SPARC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPARC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057587
Assay Design:   exonic
Chromosome Location:   5:151041636-151041785question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000231061

Gene Information

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq Dec 2011]

Gene Symbol:   SPARC
Gene Name:   secreted protein, acidic, cysteine-rich (osteonectin)
Aliases:   ON
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000113140
Entrez:   6678
Chromosome Mapping:   5q31.3-q32

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