PrimePCR™ Probe Assay: ECT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ECT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ECT2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028479
Assay Design:   Intron-spanning
Chromosome Location:   3:172520744-172523641question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000232458 ENST00000392692 ENST00000417960 ENST00000441497 ENST00000437296 ENST00000540509 ENST00000427830

Gene Information

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus this protein is expressed in a cell cycle-dependent manner during liver regeneration and is thought to have an important role in the regulation of cytokinesis. [provided by RefSeq Jul 2008]

Gene Symbol:   ECT2
Gene Name:   epithelial cell transforming sequence 2 oncogene
Aliases:   ARHGEF31, FLJ10461, MGC138291
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114346
Entrez:   1894
Chromosome Mapping:   3q26.1-q26.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 33.130000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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