PrimePCR™ Probe Assay: GUCY1B3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GUCY1B3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GUCY1B3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030883
Assay Design:   Intron-spanning
Chromosome Location:   4:156715152-156716580question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000505154 ENST00000502959 ENST00000505764 ENST00000507146 ENST00000264424 ENST00000503520 ENST00000513437

Gene Information

Soluble guanylate cyclase (sGC) a heterodimeric protein consisting of an alpha subunit and a beta subunit typically GUCY1B3 catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide (NO) and nitrovasodilator drugs (Zabel et al. 1998 [PubMed 9742212]).[supplied by OMIM Mar 2008]

Gene Symbol:   GUCY1B3
Gene Name:   guanylate cyclase 1, soluble, beta 3
Aliases:   GC-S-beta-1, GC-SB3, GUC1B3, GUCB3, GUCSB3, GUCY1B1
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000061918
Entrez:   2983
Chromosome Mapping:   4q31.3-q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.280000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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