PrimePCR™ Probe Assay: Plcb4, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Plcb4, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Plcb4, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025465
Assay Design:   intron_spanning
Chromosome Location:   3:135395019-135398464question
Amplicon Length:   117
Splice Variants Targeted:   ENSRNOT00000007247 ENSRNOT00000045393 ENSRNOT00000042853 ENSRNOT00000049855

Gene Information

phospholipase C isozyme; may play a role in phototransduction [RGD, Feb 2006]

Gene Symbol:   Plcb4
Gene Name:   1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
Aliases:   Not Available
RefSeq:   NM_024353
Ensembl:   ENSRNOG00000033119
Entrez:   25031
Chromosome Mapping:   3q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.290000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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