PrimePCR™ Probe Assay: GGCT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GGCT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GGCT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0023987
Assay Design:   Exonic
Chromosome Location:   7:30544203-30544381question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000275428 ENST00000409144 ENST00000409390 ENST00000409436 ENST00000005374

Gene Information

The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides the penultimate step in glutathione catabolism and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Dec 2010]

Gene Symbol:   GGCT
Gene Name:   gamma-glutamylcyclotransferase
Aliases:   C7orf24, CRF21, FLJ11717, GCTG, GGC, MGC3077
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000006625
Entrez:   79017
Chromosome Mapping:   7p15-p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.030000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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