PrimePCR™ Probe Assay: HADHA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HADHA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HADHA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025893
Assay Design:   Exonic
Chromosome Location:   2:26417961-26418055question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000380649 ENST00000492433

Gene Information

This gene encodes the alpha subunit of the mitochondrial trifunctional protein which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq Jul 2008]

Gene Symbol:   HADHA
Gene Name:   hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit
Aliases:   ECHA, GBP, HADH, LCEH, LCHAD, MGC1728, MTPA, TP-ALPHA
RefSeq:   NC_000002.11 NG_007121.1 NG_007294.1 NT_022184.15
Ensembl:   ENSG00000084754
Entrez:   3030
Chromosome Mapping:   2p23

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