PrimePCR™ Probe Assay: UGT1A3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: UGT1A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UGT1A3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041708
Assay Design:   exonic
Chromosome Location:   2:234638084-234638194question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000373409 ENST00000482026

Gene Information

This gene encodes a UDP-glucuronosyltransferase an enzyme of the glucuronidation pathway that transforms small lipophilic molecules such as steroids bilirubin hormones and drugs into water-soluble excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site and is regulated by its own promoter. Substrates of this enzyme include estrone 2-hydroxyestrone and metabolites of benzo alpha-pyrene. [provided by RefSeq Jul 2008]

Gene Symbol:   UGT1A3
Gene Name:   UDP glucuronosyltransferase 1 family, polypeptide A3
Aliases:   UDPGT 1-3, UGT1C
RefSeq:   NC_000002.11 NG_002601.2 NG_009254.1 NT_005120.16
Ensembl:   ENSG00000243135
Entrez:   54659
Chromosome Mapping:   2q37

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.000000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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