PrimePCR™ Probe Assay: VIM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: VIM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VIM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049297
Assay Design:   exonic
Chromosome Location:   10:17279368-17279501question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000224237 ENST00000544301

Gene Information

This gene encodes a member of the intermediate filament family. Intermediate filamentents along with microtubules and actin microfilaments make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape integrity of the cytoplasm and stabilizing cytoskeletal interactions. It is also involved in the immune response and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment migration and cell signaling. Mutations in this gene causes a dominant pulverulent cataract.[provided by RefSeq Jun 2009]

Gene Symbol:   VIM
Gene Name:   vimentin
Aliases:   FLJ36605
RefSeq:   NC_000010.10 NG_012413.1 NT_008705.16
Ensembl:   ENSG00000026025
Entrez:   7431
Chromosome Mapping:   10p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.630000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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