PrimePCR™ Probe Assay: PDLIM2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PDLIM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDLIM2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051148
Assay Design:   exonic
Chromosome Location:   8:22438203-22439021question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000567584 ENST00000569114 ENST00000564122 ENST00000591806 ENST00000344663 ENST00000311401 ENST00000308677 ENST00000264444 ENST00000362003 ENST00000427463 ENST00000470161 ENST00000433814 ENST00000505655 ENST00000276297 ENST00000358919 ENST00000512044 ENST00000520226 ENST00000356994 ENST00000320476 ENST00000377533 ENST00000301732 ENST00000382381 ENST00000456545 ENST00000308354 ENST00000452226 ENST00000397761 ENST00000436754 ENST00000426493 ENST00000429812 ENST00000409141 ENST00000265810 ENST

Gene Information

This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   PDLIM2
Gene Name:   PDZ and LIM domain 2 (mystique)
Aliases:   FLJ34715, MYSTIQUE, SLIM
RefSeq:   Not Available
Ensembl:   ENSG00000120913
Entrez:   64236
Chromosome Mapping:   8p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.020000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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