PrimePCR™ Probe Assay: BRIP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BRIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BRIP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051957
Assay Design:   exonic
Chromosome Location:   17:59821821-59821928question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000584322 ENST00000577598 ENST00000259008

Gene Information

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq Jul 2008]

Gene Symbol:   BRIP1
Gene Name:   BRCA1 interacting protein C-terminal helicase 1
Aliases:   BACH1, FANCJ, FLJ90232, MGC126521, MGC126523, OF
RefSeq:   NC_000017.10 NG_007409.2 NT_010783.15
Ensembl:   ENSG00000136492
Entrez:   83990
Chromosome Mapping:   17q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.540000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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