PrimePCR™ Probe Assay: PFKM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PFKM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PFKM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052831
Assay Design:   exonic
Chromosome Location:   12:48524146-48525127question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000550345 ENST00000549003 ENST00000550924 ENST00000549941 ENST00000550257 ENST00000340802 ENST00000549366 ENST00000552792 ENST00000548288 ENST00000359794 ENST00000551339 ENST00000548345 ENST00000551804 ENST00000549022 ENST00000547587 ENST00000395233 ENST00000312352

Gene Information

Three phosphofructokinase isozymes exist in humans: muscle liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase which catalyzes the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq Nov 2009]

Gene Symbol:   PFKM
Gene Name:   phosphofructokinase, muscle
Aliases:   GSD7, MGC8699, PFK-1, PFK1, PFKA, PFKX
RefSeq:   NC_000012.11 NT_029419.12 NG_016199.1
Ensembl:   ENSG00000152556
Entrez:   5213
Chromosome Mapping:   12q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.680000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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