PrimePCR™ Probe Assay: CLDN7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CLDN7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CLDN7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054751
Assay Design:   exonic
Chromosome Location:   17:7164162-7164284question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000544334 ENST00000306801 ENST00000538261 ENST00000571932 ENST00000571881 ENST00000575313 ENST00000574070 ENST00000397317 ENST00000360325 ENST00000565826 ENST00000483017 ENST00000329146 ENST00000529193 ENST00000288400 ENST00000262304 ENST00000423118 ENST00000217939 ENST00000442129 ENST00000392957 ENST00000395148 ENST00000260356 ENST00000320955 ENST00000255008 ENST00000461958 ENST00000377311 ENST00000303391 ENST00000368713 ENST00000375337 ENST00000304129 ENST00000369271 ENST00000545353 ENST

Gene Information

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies including breast cancer ovarian cancer hepatocellular carcinomas urinary tumors prostate cancer lung cancer head and neck cancers thyroid carcinomas etc.. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq May 2010]

Gene Symbol:   CLDN7
Gene Name:   claudin 7
Aliases:   CEPTRL2, CLDN-7, CPETRL2, Hs.84359, claudin-1
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000181885
Entrez:   1366
Chromosome Mapping:   17p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.250000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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