PrimePCR™ Probe Assay: MTA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MTA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MTA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054823
Assay Design:   exonic
Chromosome Location:   14:105936326-105936487question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000331320 ENST00000406191 ENST00000405646 ENST00000434050 ENST00000426567 ENST00000494981 ENST00000435036 ENST00000380752 ENST00000458069 ENST00000374049 ENST00000383832 ENST00000383831 ENST00000345060 ENST00000287777 ENST00000532179 ENST00000537344 ENST00000291107

Gene Information

This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells specifically mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex NuRD but it is more likely that this component is a different but very similar protein. These two proteins are so closely related though that they share the same types of domains. These domains include two DNA binding domains a dimerization domain and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:   MTA1
Gene Name:   metastasis associated 1
Aliases:   Not Available
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000182979
Entrez:   9112
Chromosome Mapping:   14q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.820000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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