PrimePCR™ Probe Assay: PTTG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTTG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTTG1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040843
Assay Design:   Intron-spanning
Chromosome Location:   5:159851301-159854825question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000280326 ENST00000515390 ENST00000504686 ENST00000352433 ENST00000520452 ENST00000393964

Gene Information

The encoded protein is a homolog of yeast securin proteins which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs which are required for its transforming and tumorigenic activities as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein although it partially localizes in the nucleus. [provided by RefSeq Jul 2008]

Gene Symbol:   PTTG1
Gene Name:   pituitary tumor-transforming 1
Aliases:   EAP1, HPTTG, MGC126883, MGC138276, PTTG, TUTR1
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000164611
Entrez:   9232
Chromosome Mapping:   5q35.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.950000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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