PrimePCR™ Probe Assay: Fgfr2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Fgfr2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Fgfr2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0056123
Assay Design:   exonic
Chromosome Location:   7:130172829-130172937question
Amplicon Length:   79
Splice Variants Targeted:   ENSMUST00000124096 ENSMUST00000117872 ENSMUST00000122054 ENSMUST00000120187 ENSMUST00000121064 ENSMUST00000117754 ENSMUST00000120715 ENSMUST00000119260 ENSMUST00000117089 ENSMUST00000117691 ENSMUST00000117858 ENSMUST00000120141 ENSMUST00000117357 ENSMUST00000122448 ENSMUST00000118296 ENSMUST00000121080 ENSMUST00000117073

Gene Information

Description Not Available

Gene Symbol:   Fgfr2
Gene Name:   Fibroblast growth factor receptor 2
Aliases:   AU043015, AW556123, Bek, Fgfr-2, Fgfr-7, Fgfr7, KGFR, KGFRTr, svs
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000030849
Entrez:   14183
Chromosome Mapping:   7 F3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.590000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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