PrimePCR™ Probe Assay: Acadm, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Acadm, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Acadm, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030845
Assay Design:   Intron-spanning
Chromosome Location:   3:153922879-153925690question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000072697

Gene Information

This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple intronless pseudogenes. [provided by RefSeq Nov 2012]

Gene Symbol:   Acadm
Gene Name:   Acyl-Coenzyme A dehydrogenase, medium chain
Aliases:   AU018656, MCAD
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000062908
Entrez:   11364
Chromosome Mapping:   3 H3|3 73.6 cM

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