PrimePCR™ Probe Assay: Cttn, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Cttn, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cttn, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0024325
Assay Design:   intron_spanning
Chromosome Location:   1:224463488-224464639question
Amplicon Length:   96
Splice Variants Targeted:   ENSRNOT00000023861 ENSRNOT00000033827 ENSRNOT00000033919 ENSRNOT00000041034 ENSRNOT00000011290 ENSRNOT00000059974 ENSRNOT00000041988 ENSRNOT00000011371 ENSRNOT00000071529 ENSRNOT00000066774 ENSRNOT00000020768 ENSRNOT00000010895 ENSRNOT00000051590 ENSRNOT00000040845 ENSRNOT00000030344 ENSRNOT00000015181 ENSRNOT00000026448 ENSRNOT00000030037 ENSRNOT00000027836 ENSRNOT00000028283 ENSRNOT00000047669 ENSRNOT00000054859 ENSRNOT00000020763

Gene Information

component of clathrin-coated pits; involved in receptor-mediated endocytosis [RGD, Feb 2006]

Gene Symbol:   Cttn
Gene Name:   Cortactin; Cortactin, isoform CRA_e; Protein Cttn
Aliases:   Not Available
RefSeq:   NM_021868
Ensembl:   ENSRNOG00000047280
Entrez:   60465
Chromosome Mapping:   1q41-q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 41.310000
Efficiency 82

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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