PrimePCR™ Probe Assay: IL17RC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL17RC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL17RC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0023985
Assay Design:   Exonic
Chromosome Location:   3:9965592-9965691question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000383812 ENST00000438091 ENST00000295981 ENST00000436503 ENST00000403601 ENST00000455057 ENST00000413608 ENST00000416074

Gene Information

This gene encodes a single-pass type I membrane protein that shares similarity with the interleukin-17 receptor (IL-17RA). Unlike IL-17RA which is predominantly expressed in hemopoietic cells and binds with high affinity to only IL-17A this protein is expressed in nonhemopoietic tissues and binds both IL-17A and IL-17F with similar affinities. The proinflammatory cytokines IL-17A and IL-17F have been implicated in the progression of inflammatory and autoimmune diseases. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene and it has been proposed that soluble secreted proteins lacking transmembrane and intracellular domains may function as extracellular antagonists to cytokine signaling. [provided by RefSeq Feb 2011]

Gene Symbol:   IL17RC
Gene Name:   interleukin 17 receptor C
Aliases:   FLJ95963, FLJ96005, IL17-RL, IL17RL, MGC10763
RefSeq:   NG_017069.1 NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163702
Entrez:   84818
Chromosome Mapping:   3p25.3,3p25.3-p24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.570000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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