PrimePCR™ Probe Assay: EIF4G1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EIF4G1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EIF4G1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024464
Assay Design:   Exonic
Chromosome Location:   3:184040643-184040891question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000346169 ENST00000414031 ENST00000392537 ENST00000450424 ENST00000421110 ENST00000382330 ENST00000426123 ENST00000350481 ENST00000427845 ENST00000342981 ENST00000424196 ENST00000411531 ENST00000444861 ENST00000441154 ENST00000434061 ENST00000435046 ENST00000352767 ENST00000319274

Gene Information

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants some of which are derived from alternative promoter usage. [provided by RefSeq Aug 2010]

Gene Symbol:   EIF4G1
Gene Name:   eukaryotic translation initiation factor 4 gamma, 1
Aliases:   DKFZp686A1451, EIF-4G1, EIF4F, EIF4G, EIF4GI, P220
RefSeq:   NC_000003.11 NT_005612.16 NG_016850.1
Ensembl:   ENSG00000114867
Entrez:   1981
Chromosome Mapping:   3q27-qter

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.380000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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