PrimePCR™ Probe Assay: CREM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CREM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CREM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040673
Assay Design:   exonic
Chromosome Location:   10:35495823-35495937question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000579455 ENST00000382675 ENST00000474362 ENST00000374721 ENST00000429130 ENST00000460270 ENST00000354759 ENST00000345491 ENST00000487132 ENST00000337656 ENST00000374734 ENST00000479070 ENST00000348787 ENST00000361599 ENST00000484283 ENST00000395887 ENST00000494479 ENST00000463314 ENST00000342105 ENST00000463960 ENST00000487763 ENST00000473940 ENST00000488328 ENST00000356917 ENST00000488741 ENST00000474931 ENST00000468236 ENST00000344351 ENST00000490511 ENST00000395895 ENST00000333809 ENST

Gene Information

This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq Jul 2008]

Gene Symbol:   CREM
Gene Name:   cAMP responsive element modulator
Aliases:   ICER, MGC111110, MGC17881, MGC41893, hCREM-2
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000095794
Entrez:   1390
Chromosome Mapping:   10p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.140000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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