PrimePCR™ Probe Assay: AK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040700
Assay Design:   exonic
Chromosome Location:   9:130630600-130630761question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000373176 ENST00000413016 ENST00000373156 ENST00000223836

Gene Information

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates adenylate isozyme 1 (AK1) 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle brain and erythrocytes whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq Jul 2008]

Gene Symbol:   AK1
Gene Name:   adenylate kinase 1
Aliases:   Not Available
RefSeq:   NC_000009.11 NG_011792.1 NT_008470.19
Ensembl:   ENSG00000106992
Entrez:   203
Chromosome Mapping:   9q34.1

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