PrimePCR™ Probe Assay: AKR1C3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AKR1C3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AKR1C3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040990
Assay Design:   exonic
Chromosome Location:   10:5139646-5141008question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000263026 ENST00000523374 ENST00000380872 ENST00000442997 ENST00000380859 ENST00000434459 ENST00000380753 ENST00000421196 ENST00000407674 ENST00000455190 ENST00000380554 ENST00000439082 ENST00000380448 ENST00000263126 ENST00000340740 ENST00000398823 ENST00000578362 ENST00000582412 ENST00000578631 ENST00000584844 ENST00000580545 ENST00000578591 ENST00000580345 ENST00000585272 ENST00000585191 ENST00000583876 ENST00000579965 ENST00000583238

Gene Information

This gene encodes a member of the aldo/keto reductase superfamily which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2 PGH2 and phenanthrenequinone (PQ) and the oxidation of 9alpha11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   AKR1C3
Gene Name:   aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
Aliases:   DD3, DDX, HA1753, HAKRB, HAKRe, HSD17B5, KIAA0119, PGFS, hluPGFS
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000196139
Entrez:   8644
Chromosome Mapping:   10p15-p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.730000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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