PrimePCR™ Probe Assay: COMT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COMT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COMT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049931
Assay Design:   exonic
Chromosome Location:   22:19950146-19950236question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000418115 ENST00000370196 ENST00000467928 ENST00000513317 ENST00000594671 ENST00000262960 ENST00000598800 ENST00000597849 ENST00000597726 ENST00000444743 ENST00000260867 ENST00000374065 ENST00000374064 ENST00000361682 ENST00000403184 ENST00000403710 ENST00000407537 ENST00000412786 ENST00000406520 ENST00000449653

Gene Information

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines including the neurotransmitters dopamine epinephrine and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances COMT is important in the metabolism of catechol drugs used in the treatment of hypertension asthma and Parkinson disease. COMT is found in two forms in tissues a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq Sep 2008]

Gene Symbol:   COMT
Gene Name:   catechol-O-methyltransferase
Aliases:   Not Available
RefSeq:   NC_000022.10 NG_011526.1 NG_011835.1 NG_023326.1 NT_011519.10
Ensembl:   ENSG00000093010
Entrez:   1312
Chromosome Mapping:   22q11.21-q11.23,22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.720000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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