PrimePCR™ Probe Assay: PDGFD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PDGFD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDGFD, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031023
Assay Design:   Intron-spanning
Chromosome Location:   11:103866883-103870858question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000393158 ENST00000302251 ENST00000529268

Gene Information

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines seven of which are found in this factor. This gene product only forms homodimers and therefore does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PDGFD
Gene Name:   platelet derived growth factor D
Aliases:   IEGF, MGC26867, SCDGF-B, SCDGFB
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000170962
Entrez:   80310
Chromosome Mapping:   11q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.930000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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