PrimePCR™ Probe Assay: NCOA3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NCOA3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCOA3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031885
Assay Design:   Intron-spanning
Chromosome Location:   20:46262886-46264129question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000372004 ENST00000371998 ENST00000371997 ENST00000340189 ENST00000341724 ENST00000542882

Gene Information

The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq Mar 2010]

Gene Symbol:   NCOA3
Gene Name:   nuclear receptor coactivator 3
Aliases:   ACTR, AIB-1, AIB1, CAGH16, CTG26, KAT13B, MGC141848, RAC3, SRC-3, SRC3, TNRC14, TNRC16, TRAM-1, bHLHe42, pCIP
RefSeq:   NC_000020.10 NT_011362.10 NG_016810.1
Ensembl:   ENSG00000124151
Entrez:   8202
Chromosome Mapping:   20q12

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