PrimePCR™ Probe Assay: ALDH1A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ALDH1A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ALDH1A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031939
Assay Design:   Intron-spanning
Chromosome Location:   9:75543912-75545863question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000297785 ENST00000376939 ENST00000419959 ENST00000446946 ENST00000428593

Gene Information

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver cytosolic and mitochondrial which are encoded by distinct genes and can be distinguished by their electrophoretic mobility kinetic properties and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq Mar 2011]

Gene Symbol:   ALDH1A1
Gene Name:   aldehyde dehydrogenase 1 family, member A1
Aliases:   ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1
RefSeq:   NC_000009.11 NG_012249.1 NT_008470.19
Ensembl:   ENSG00000165092
Entrez:   216
Chromosome Mapping:   9q21.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.760000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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