PrimePCR™ Probe Assay: FKBP1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FKBP1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FKBP1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041373
Assay Design:   Intron-spanning
Chromosome Location:   20:1352778-1356176question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000445888 ENST00000420246 ENST00000455263 ENST00000269305 ENST00000356170 ENST00000370659 ENST00000252444 ENST00000370275 ENST00000460006 ENST00000400137 ENST00000381724 ENST00000381719 ENST00000381715 ENST00000439640 ENST00000325718 ENST00000318217 ENST00000394649 ENST00000254325 ENST00000368073 ENST00000326837 ENST00000368074 ENST00000321753 ENST00000439986 ENST00000523767 ENST00000261797 ENST00000371929 ENST00000371916 ENST00000262325 ENST00000592545 ENST00000312678 ENST00000589344 ENST

Gene Information

The protein encoded by this gene is a member of the immunophilin protein family which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants encoding the same protein have been identified. The human genome contains five pseudogenes related to this gene at least one of which is transcribed. [provided by RefSeq Sep 2008]

Gene Symbol:   FKBP1A
Gene Name:   FK506 binding protein 1A, 12kDa
Aliases:   FKBP-12, FKBP1, FKBP12, PKC12, PKCI2, PPIASE
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000088832
Entrez:   2280
Chromosome Mapping:   20p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.340000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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