PrimePCR™ Probe Assay: CFLAR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CFLAR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CFLAR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041627
Assay Design:   Intron-spanning
Chromosome Location:   2:201997826-202000808question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000309955 ENST00000443227 ENST00000341222 ENST00000341582 ENST00000342795 ENST00000395148 ENST00000423241 ENST00000440180 ENST00000457277 ENST00000494258 ENST00000470178 ENST00000462763 ENST00000479953 ENST00000355558 ENST00000340870

Gene Information

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene and partial evidence for several more variants exists. [provided by RefSeq Feb 2011]

Gene Symbol:   CFLAR
Gene Name:   CASP8 and FADD-like apoptosis regulator
Aliases:   CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000003402
Entrez:   8837
Chromosome Mapping:   2q33-q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.820000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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