PrimePCR™ Probe Assay: RP11-155D18.11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RP11-155D18.11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RP11-155D18.11, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039794
Assay Design:   exonic
Chromosome Location:   3:52023035-52023155question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000340908 ENST00000530271 ENST00000537687 ENST00000315939 ENST00000535572 ENST00000300648 ENST00000534313 ENST00000527525 ENST00000394224 ENST00000394227 ENST00000445101 ENST00000431721 ENST00000446860 ENST00000443964 ENST00000332271 ENST00000263253 ENST00000522555 ENST00000522236 ENST00000316059 ENST00000263801 ENST00000382039 ENST00000382044 ENST00000450115 ENST00000522455 ENST00000518566 ENST00000517638 ENST00000523850 ENST00000521376 ENST00000476854 ENST00000476351 ENST00000494103 ENST

Gene Information

This gene encodes a cytosolic homodimeric zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1 a region reduced to homozygosity in small-cell lung cancer (SCLC) and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq Nov 2010]

Gene Symbol:   RP11-155D18.11
Gene Name:   Aminoacylase-1
Aliases:   ACY-1, ACY1D
RefSeq:   NC_000003.11 NG_012036.1 NT_022517.18
Ensembl:   ENSG00000114786
Entrez:   95
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.230000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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