PrimePCR™ Probe Assay: ABCB11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ABCB11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ABCB11, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049601
Assay Design:   exonic
Chromosome Location:   2:169779523-169779669question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000576612 ENST00000378623 ENST00000522555 ENST00000522236 ENST00000263817 ENST00000431162 ENST00000440121 ENST00000301819 ENST00000270861 ENST00000513090 ENST00000507249 ENST00000514379 ENST00000360156 ENST00000354228 ENST00000489216 ENST00000354416 ENST00000343984 ENST00000394610 ENST00000394617 ENST00000394616 ENST00000314328 ENST00000437398

Gene Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq Jul 2008]

Gene Symbol:   ABCB11
Gene Name:   ATP-binding cassette, sub-family B (MDR/TAP), member 11
Aliases:   ABC16, BRIC2, BSEP, PFIC-2, PFIC2, PGY4, SPGP
RefSeq:   NC_000002.11 NG_007374.1 NT_005403.17 NW_003315909.1
Ensembl:   ENSG00000073734
Entrez:   8647
Chromosome Mapping:   2q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.980000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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