PrimePCR™ Probe Assay: ASMT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ASMT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ASMT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055345
Assay Design:   exonic
Chromosome Location:   X:1761787-1761888question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000381241 ENST00000381229 ENST00000381233 ENST00000432523

Gene Information

This gene belongs to the methyltransferase superfamily and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq Jan 2010]

Gene Symbol:   ASMT
Gene Name:   acetylserotonin O-methyltransferase
Aliases:   ASMTY, HIOMT, HIOMTY
RefSeq:   NC_000023.10 NC_000024.9 NT_167196.1 NT_167204.1 NG_016337.1 NG_021470.1
Ensembl:   ENSG00000196433
Entrez:   438
Chromosome Mapping:   Xp22.3 or Yp11.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.100000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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