PrimePCR™ Probe Assay: POLD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: POLD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POLD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026846
Assay Design:   Intron-spanning
Chromosome Location:   19:50902695-50905161question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000376930 ENST00000440232

Gene Information

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq Mar 2012]

Gene Symbol:   POLD1
Gene Name:   polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Aliases:   CDC2, POLD
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000062822
Entrez:   5424
Chromosome Mapping:   19q13.3

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