PrimePCR™ Probe Assay: ZNF331, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF331, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF331, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028994
Assay Design:   Intron-spanning
Chromosome Location:   19:54059144-54074943question
Amplicon Length:   157
Splice Variants Targeted:   ENST00000253144 ENST00000511593 ENST00000502248 ENST00000514374 ENST00000411977 ENST00000511154 ENST00000509047 ENST00000509585 ENST00000513999 ENST00000512387 ENST00000511567 ENST00000514022 ENST00000505949 ENST00000513265 ENST00000502616 ENST00000504493 ENST00000505426

Gene Information

This gene encodes a zinc finger protein containing a KRAB (Kruppel-associated box) domain found in transcriptional repressors. A pseudogene of this gene is located on chromosome 17. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Dec 2011]

Gene Symbol:   ZNF331
Gene Name:   zinc finger protein 331
Aliases:   DKFZp686L0787, RITA, ZNF361, ZNF463
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000130844
Entrez:   55422
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.080000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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