PrimePCR™ Probe Assay: ROBO2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ROBO2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ROBO2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029636
Assay Design:   Intron-spanning
Chromosome Location:   3:77607166-77611835question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000487694 ENST00000461745 ENST00000332191 ENST00000403211 ENST00000343019 ENST00000398467

Gene Information

This gene belongs to the ROBO family part of the immunoglobulin superfamily proteins that are highly conserved from fly to human. The encoded protein is a receptor for SLIT2 molecules known to function in axon guidance and cell migration. Defects in this gene are the cause of vesicoureteral reflux type 2. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ROBO2
Gene Name:   roundabout, axon guidance receptor, homolog 2 (Drosophila)
Aliases:   KIAA1568, SAX3
RefSeq:   NC_000003.11 NG_027734.1 NT_022459.15
Ensembl:   ENSG00000185008
Entrez:   6092
Chromosome Mapping:   3p12.3

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