PrimePCR™ lncRNA SYBR® Green Assay:DLAT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0181466

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: DLAT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: DLAT, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0104226
Assay Design:   exonic
Chromosome Location:   chr11:112025543-112025640question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000280346 ENST00000393051 ENST00000533297
LNCipedia Splice Variants Targeted:   lnc-DIXDC1-2:1

Gene Information

This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene dihydrolipoamide acetyltransferase accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq Oct 2009]

Gene Symbol:   DLAT
Gene Name:   dihydrolipoamide S-acetyltransferase
Aliases:   DLTA, PDC-E2, PDCE2
RefSeq:   NC_000011.9 NG_013342.1 NT_033899.8
Ensembl:   ENSG00000150768
LNCipedia:   lnc-DIXDC1-2
Entrez:   1737
Chromosome Mapping:   11q23.1
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download