PrimePCR™ lncRNA® Probe Assay:H19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLED0074346

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: H19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: H19, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLEP0151586
Assay Design:   exonic
Chromosome Location:   chr11:1995992-1996180question
Amplicon Length:   79
Refseq Transcripts Amplified:   NR_131224,NR_131223,NR_002196
Splice Variants Targeted:   ENST00000414790 ENST00000446406 ENST00000436715 ENST00000439725 ENST00000442037 ENST00000417089 ENST00000428066 ENST00000447298 ENST00000431095 ENST00000411861 ENST00000411754 ENST00000412788
LNCipedia Splice Variants Targeted:   H19:3 H19:4 H19:6 H19:9 H19:1 H19:5 H19:7 H19:12 H19:17 H19:14 H19:2 H19:16 H19:15 H19:8 H19:13 H19:10

Gene Information

Not Available

Gene Symbol:   H19
Gene Name:   H19, imprinted maternally expressed transcript (non-protein coding) [Source:HGNC Symbol;Acc:HGNC:4713]
Aliases:   ASM, ASM1, BWS, D11S813E, LINC00008, NCRNA00008, WT2
RefSeq:   Not Available
Ensembl:   ENSG00000130600
LNCipedia:   H19
Entrez:   283120
Chromosome Mapping:   11p15.5
Gene Strand:   Reverse strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.689999
Efficiency 1.96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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