PrimePCR™ Probe Assay: BRCA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BRCA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BRCA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041326
Assay Design:   exonic
Chromosome Location:   17:41251816-41256152question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000357654 ENST00000468300 ENST00000493795 ENST00000471181 ENST00000491747 ENST00000484087 ENST00000478531 ENST00000493919 ENST00000487825 ENST00000470026 ENST00000477152 ENST00000494123 ENST00000473961 ENST00000476777 ENST00000354071 ENST00000352993 ENST00000346315 ENST00000351666 ENST00000393691 ENST00000309486

Gene Information

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors DNA damage sensors and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II and through the C-terminal domain also interacts with histone deacetylase complexes. This protein thus plays a role in transcription DNA repair of double-stranded breaks and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants some of which are disease-associated mutations have been described for this gene but the full-length natures of only some of these variants has been described. A related pseudogene which is also located on chromosome 17 has been identified. [provided by RefSeq May 2009]

Gene Symbol:   BRCA1
Gene Name:   breast cancer 1, early onset
Aliases:   BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PSCP, RNF53
RefSeq:   NC_000017.10 NG_005905.2 NT_010783.15
Ensembl:   ENSG00000012048
Entrez:   672
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.990000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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