PrimePCR™ Probe Assay: POLR2E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: POLR2E, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POLR2E, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049979
Assay Design:   exonic
Chromosome Location:   19:1088207-1088323question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000215587

Gene Information

This gene encodes the fifth largest subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq Jul 2008]

Gene Symbol:   POLR2E
Gene Name:   polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
Aliases:   RPABC1, RPB5, XAP4, hRPB25, hsRPB5
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000099817
Entrez:   5434
Chromosome Mapping:   19p13.3

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