PrimePCR™ Probe Assay: CTSB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CTSB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CTSB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053506
Assay Design:   exonic
Chromosome Location:   8:11708397-11708486question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000539851 ENST00000370563 ENST00000263723 ENST00000353047 ENST00000530640 ENST00000531089 ENST00000453527 ENST00000345125 ENST00000533455 ENST00000534510 ENST00000534636 ENST00000533572 ENST00000530296 ENST00000526195 ENST00000527243 ENST00000534149 ENST00000526645 ENST00000505496 ENST00000524500 ENST00000531502 ENST00000434271 ENST00000415599 ENST00000374314 ENST00000354618 ENST00000469141 ENST00000575926

Gene Information

The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease the most common cause of dementia. Overexpression of the encoded protein which is a member of the peptidase C1 family has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CTSB
Gene Name:   cathepsin B
Aliases:   APPS, CPSB
RefSeq:   NC_000008.10 NT_077531.4 NG_009217.1
Ensembl:   ENSG00000164733
Entrez:   1508
Chromosome Mapping:   8p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.050000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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