PrimePCR™ Probe Assay: CHEK2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CHEK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHEK2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029419
Assay Design:   Intron-spanning
Chromosome Location:   22:29107897-29115459question
Amplicon Length:   156
Splice Variants Targeted:   ENST00000328354 ENST00000404276 ENST00000405598 ENST00000382580 ENST00000403642 ENST00000402731 ENST00000425190 ENST00000439200 ENST00000348295 ENST00000382578 ENST00000544772 ENST00000382566

Gene Information

In response to DNA damage and replication blocks cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated the encoded protein is known to inhibit CDC25C phosphatase preventing entry into mitosis and has been shown to stabilize the tumor suppressor protein p53 leading to cell cycle arrest in G1. In addition this protein interacts with and phosphorylates BRCA1 allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also mutations in this gene are thought to confer a predisposition to sarcomas breast cancer and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2012]

Gene Symbol:   CHEK2
Gene Name:   CHK2 checkpoint homolog (S. pombe)
Aliases:   CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53
RefSeq:   NC_000022.10 NG_008150.1 NT_011520.12
Ensembl:   ENSG00000183765
Entrez:   11200
Chromosome Mapping:   22q11,22q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.130000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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