PrimePCR™ Probe Assay: ST8SIA3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ST8SIA3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ST8SIA3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030146
Assay Design:   Intron-spanning
Chromosome Location:   18:55020154-55021677question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000541833 ENST00000324000

Gene Information

ST8SIA3 belongs to a family of sialyltransferases that form sialyl-alpha-28-sialyl-R linkages at the nonreducing termini of glycoconjugates (Lee et al. 1998 [PubMed 9826427]).[supplied by OMIM Mar 2008]

Gene Symbol:   ST8SIA3
Gene Name:   ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Aliases:   SIAT8C, ST8SiaIII
RefSeq:   NC_000018.9 NT_025028.14
Ensembl:   ENSG00000177511
Entrez:   51046
Chromosome Mapping:   18q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.410000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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