PrimePCR™ Probe Assay: ALPP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ALPP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ALPP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032633
Assay Design:   Intron-spanning
Chromosome Location:   2:233246049-233246361question
Amplicon Length:   154
Splice Variants Targeted:   ENST00000392027

Gene Information

There are at least four distinct but related alkaline phosphatases: intestinal placental placental-like and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme also referred to as the heat stable form that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition this gene is polymorphic and three common alleles (type 1 type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   ALPP
Gene Name:   alkaline phosphatase, placental
Aliases:   ALP, FLJ61142, PALP, PLAP
RefSeq:   NC_000002.11 NG_012189.1 NT_005403.17
Ensembl:   ENSG00000163283
Entrez:   250
Chromosome Mapping:   2q37

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