PrimePCR™ Probe Assay: LLGL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LLGL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LLGL2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040476
Assay Design:   Intron-spanning
Chromosome Location:   17:73539561-73554264question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000580578 ENST00000167462 ENST00000375227 ENST00000392550 ENST00000578363 ENST00000579392 ENST00000577200 ENST00000578536 ENST00000581713

Gene Information

The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division epithelial cell polarity and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   LLGL2
Gene Name:   lethal giant larvae homolog 2 (Drosophila)
Aliases:   HGL, LGL2
RefSeq:   NC_000017.10 NG_013041.1 NT_010783.15
Ensembl:   ENSG00000073350
Entrez:   3993
Chromosome Mapping:   17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.710000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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