PrimePCR™ Probe Assay: MDC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MDC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MDC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039817
Assay Design:   exonic
Chromosome Location:   6:30681837-30681929question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000543601 ENST00000285046 ENST00000378313 ENST00000470681 ENST00000376406 ENST00000416571 ENST00000425072 ENST00000422266 ENST00000376405 ENST00000440369 ENST00000425029 ENST00000417228 ENST00000424638 ENST00000548542 ENST00000547047 ENST00000548248 ENST00000550500 ENST00000427406 ENST00000424507 ENST00000438165 ENST00000456589 ENST00000547874 ENST00000551460 ENST00000547700 ENST00000552522 ENST00000409674 ENST00000315392 ENST00000444911 ENST00000539608 ENST00000435664 ENST00000412395 ENST

Gene Information

The protein encoded by this gene contains an N-terminal forkhead domain two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq Jul 2008]

Gene Symbol:   MDC1
Gene Name:   mediator of DNA-damage checkpoint 1
Aliases:   DKFZp781A0122, KIAA0170, MGC166888, NFBD1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000137337
Entrez:   9656
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.070000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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